Figure 6

Delayed treatment with plumbagin protects mice from sepsis. (A) The cecal ligation and puncture (CLP) technique was used to induce intraabdominal sepsis in mice (n = 20/group). Repeated administration of plumbagin (4 mg/kg) at 24, 48, and 72 h after CLP significantly increased survival compared with vehicle group (*, p < 0.05), as measured by Kaplan-Meier test. (B-G) In parallel experiments, serum levels of lactate (B), IL-1β (F), HMGB1 (C) and PKM2 mRNA (D), NOX activity (E) and ECAR (G) in isolated peritoneal macrophages at indicated time points were measured (n = 3–5 animals/group, values are mean ± SEM, *, p < 0.05).