Figure 2

Expression pattern of PPARs and other adipose markers in perigonadal and subcutaneous WAT depots during pregnancy. (A and C) mRNA levels of adipogenic markers and other genes in (A) perigonadal and (C) subcutaneous fat depots from pregnant and nonpregnant WT and PPγ2KO mice. Data are expressed as mean ± SEM (n = 6–8 animals/group). (B and C) Quantification of PPARγ1 and PPARγ2 total protein expression in perigonadal (B) and subcutaneous (D) fat depots from pregnant and nonpregnant WT and PPγ2KO mice. Levels of protein were normalized to total β-actin. Data are expressed as mean ± SEM (n = 3–5 animals/group). *p ≤ .05; **** p ≤ .001 PPγ2KO-P versus WT-P; # p ≤ .05; ###p ≤ .001 PPγ2KO-P versus WT-C; † p ≤ .05; ††† p ≤ .001 pregnant versus non pregnant; § p ≤ .05 PPγ2KO-C versus WT-C.