Figure 3

Role of PPARγ2 in fat storage and mobilization in perigonadal and subcutaneous WAT depots during pregnancy. (A and C) mRNA levels of lipogenic and lipolytic genes in (A) perigonadal and (C) subcutaneous fat depots from pregnant and nonpregnant WT and PPγ2KO mice. Data are expressed as mean ± SEM (n = 6–8 animals/group). Total protein quantification of lipolytic enzymes ATGL and HSL in homogenates from (B) perigonadal and (D) subcutaneous fat depots from pregnant and nonpregnant WT and PPγ2KO mice. Levels of protein were normalized to total β-actin. Data are expressed as mean ± SEM (n = 3–5 animals/group). *p ≤ .05 PPγ2KO-P versus WT-P; #p ≤ .05; ###p ≤ .001 PPγ2KO-P versus WT-C; † p ≤ .05; †† p ≤ .01 pregnant versus non pregnant; § p ≤ .05 PPγ2KO-C versus WT-C.