Figure 4

Increased inflammation in perigonadal but not subcutaneous WAT in pregnant mice lacking PPARγ2. (A) mRNA levels of representative inflammatory genes in perigonadal and subcutaneous fat depots from pregnant and nonpregnant WT and PPγ2KO mice. Data are expressed as mean ± SEM (n = 6–8 animals/group). † p ≤ .05; ††† p ≤ .001 pregnant versus nonpregnant; # p ≤ .05 ###p ≤ .001 PPγ2KO-P versus WT-C; * p ≤ .05 *** p ≤ .001 PPγ2KO-P versus WT-P; § p ≤ .05 PPγ2KO-C versus WT-C. (B) Immunohistochemical analysis of MCP-1 expression in paraffin-embedded perigonadal and subcutaneous fat depots from pregnant WT and PPγ2KO groups (n = 4 animals/group). No immunoreaction was observed in the negative control treated with PBS without primary antibody. Arrows indicate the locations of infiltrated macrophages, which form crown-like structures surrounding dead and dying adipocytes, which express MCP-1. Original magnification × 200. Scale bar: 100 µm.