Figure 2

ARA290 increases insulin secretion from GK rat islets. (A) Insulin secretion expressed as µU/islet/h is measured in batch incubations of 3 W rat islets. Islets were treated with 1, 5 or 10 ng/mL of ARA290 as indicated with either 3.3 mmol/L G (□) or 16.7 mmol/L G (■). (B) Insulin secretion expressed as µU/islet/h is measured in batch incubations of 3 GK rat islets. Islets were treated with 1, 5 or 10 ng/mL of ARA290 as indicated in the graph in either 3.3 mmol/L G (□) or 16.7 mmol/L G (■). Results represent means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus 16.7 mmol/L G, ns indicates no statistical difference. (C) Dynamic glucose-stimulated insulin secretion is assessed by islets perifusion. Batches of 50 islets were exposed to 3.3 mmol/L G for 15 min, and then for an additional 15 min to 3.3 mmol/L G alone (○ curve) or plus 10 ng/mL ARA290 (● curve), followed by stimulation with 16.7 mmol/L G alone (○ curve) or plus 10 ng/mL ARA290 (● curve) for 40 min, islets were then subjected to an additional stimulation with 30 mmol/L KCL for 20 min, finally perifusion medium was changed to 3.3 mmol/L G for an additional 20 min. Results represent means ± SEM. *P < 0.05, **P < 0.01 versus control.