Figure 3

Effect of ARA290 on glucose metabolism in GK rat Islets. (A) After 30 min preincubation of islets in KRB supplemented with 3.3 mmol/L G, glucose oxidation was measured in batches of 10 GK islets during incubation for 2 h with 3.3 or 16.7 mmol/L G alone (□) or in the presence of 10 ng/mL ARA290 (■). Results represent means ± SEM of n = 6 experiments. ^#P < 0.05 versus 3.3 mmol/L G control, *P < 0.02 versus 16.7 mmol/L G control. (B) Islets were preincubated for 30 min in KRB supplemented with 3.3 mmol/L G, batches of 20 islets were the incubated for 1 h with 3.3 or 16.7 mmol/L G alone (□) or in the presence of 10 ng/mL ARA290 (■) and ATP content was measured. Results are expressed as pmole of ATP normalized by the total protein content of islets. Results represent means ± SEM of n = 6 experiments. ^#P < 0.05 versus 3.3 mmol/L G control, *P < 0.05 versus 16.7 mmol/L G control.