Figure 4

PTX3 expression is required for timely termination of the muscle regenerative response. Kinetic expression of MyoD (left) and myogenin (right) was evaluated by (A) RT-PCR or (B) Western blot in quadriceps muscles of WT (black line) and PTX3^−/− mice (dashed line) after CTX injection. n ≥ 12 mice (two-three mice/time point for WT and PTX3^−/− mice; analyses were performed in triplicate). Three independent experimental cohorts were studied. (C) The myofiber cross-sectional area (CSA) was compared in muscle of WT and PTX3^−/− mice retrieved 7, 10, 15 and 30 d after injury. n ≥ 9 mice; three independent experimental cohorts were studied. Graphs show mean values ± (A, B) s.e.m or (C) s.d.