Figure 5

Accumulation of myofiber remnants and muscle infiltration by macrophages in the absence of PTX3. Quadriceps sections of mouse muscle were analyzed 15 d after injury. Representative images of cleaved caspase 3 in (A) WT and/or (B) PTX3^−/− mice assessed by immunohistochemistry. (C) Number of cells expressing cleaved caspase 3 per FOV for WT and/or PTX3^−/− mice. Representative images for CD68 in (D) WT and/or (E) PTX3^−/− muscle assessed by immunohistochemistry. (F) Number of cells expressing CD68 per FOV for WT and/or PTX3^−/− mice. n ≥ 9. Three independent experimental cohorts were studied. Graphs show mean values ± s.d.***: significantly different from WT mice, P < .005 (Student t test). Scale bars = 20 µm.