Figure 1

Protease activity of purified Hxs. Enzymatic activity of different purified Hxs (two human and one rabbit) was tested at the same molar concentration (6.7 µmol/L) using chromogenic substrate I: S2238 and II: S2288 that are sensitive to thrombin and serine proteases respectively. Trypsin (0.4 µmol/L) was used as positive control. The substrates were mixed with different Hxs or controls and incubated at 37°C for different times. The absorbance at 405 nm was measured by spectrophotometry. The results represent mean ± SE and are representative of three independent experiments.