Figure 2
From: Purified and Recombinant Hemopexin: Protease Activity and Effect on Neutrophil Chemotaxis
Purified Hxs do not inhibit the chemotaxis of neutrophils induced by fMLF and LTB4. Human neutrophils were preincubated with different purified Hxs (hHx-A, rabbit Hx and hHx-B) or control (RPMI 1640 containing 2% FBS) for 1 h. Then 1–2.5 × 105 cells/30 µl cells were applied to the top of the transwell with the lower microplate filled with fMLF (1 nmol/L and 10 nmol/L) (A) or LTB4 (0.1 nmol/L, 1 nmol/L and 10 nmol/L) (B) and incubated for 1 h. Neutrophils migrated to the bottom microplate wells were stained with 0.4% trypan blue and live cells were counted. The percentage of cells migrated over the total cells uploaded to the top of the transwell was calculated. The results represent the mean ± SE and are representative of three independent experiments.