Figure 6
From: Purified and Recombinant Hemopexin: Protease Activity and Effect on Neutrophil Chemotaxis
Hx does not suppress neutrophil migration in mice. Thirty minutes after intravenous injection of human Hx (hHx-B) or albumin (hALB) (2 mg/mouse), neutrophil migration was induced by i.p. injection of 500 µl of 4% thioglycolate. At 2 h or 4 h post injection, peritoneal cells were flushed out by PBS and counted. (A) The peritoneal cells were incubated with PE- conjugated anti-Gr-1 and analyzed via FACS to determine the percentage of neutrophils over the total cells. Total neutrophils were calculated. (B) The MPO activity was measured in the total peritoneal cells. The results are representative of five independent experiments and presented as the mean ± SE.