Figure 5
DMA prevents LPS-stimulated IκBα degradation but does not affect levels of MAPK pathway proteins in RAW 264.7 cells. (A) Time profile analysis of IkBα degradation was determined by immunoblot of IkBα from whole-cell lysate of RAW 264.7 cells at various time points after incubation with LPS (1 µg mL−1). (B) The effect of various concentrations of DMA on IkBα degradation was determined in the presence or absence of LPS for 15 min. (C) Graphical summary of the relative densities of IkBα expression in panel B quantified using ImageJ software. (D) Immunoblots of downstream proteins of the MAPK pathway after incubation with DMA in the presence of LPS. Representative immunoblots are shown and data are means ± SEM from three independent experiments. ** P ≤ 0.01; **** P ≤ 0.0001.