Figure 1

The lentiviral vector efficiently transduced TCRγ4δ1 into peripheral blood-derived αβT cells. (A) Schematic diagram of TCRγ4δ1 receptor. The V region of TCRδ1 was amplified using RNA from the γδTILs of gastric carcinoma tissues by RT-PCR. A high-frequency CDR3δ1 sequence, termed GTM, was identified by sequence analysis. The full-length TCRδ1 with GTM and TCRγ4 chains were amplified from cDNA of one case of gastric tumor-derived γδTILs by PCR and paired to form TCRγ4δ1. (B) Schematic diagram of the lentiviral vector containing the TCRδ1 and TCRγ4 genes. The TCRδ1 and TCRγ4 genes were amplified by PCR and co-cloned into pCDH lentiviral vector. (LTR, long terminal repeats; CMV, cytomegalovirus promoter.) (C) The cell-surface expression of TCRγ4δ1 in αβT cells. Peripheral blood-derived αβT cells were lentivirally transduced with either mock lentiviral vectors or lentiviral vectors with TCRγ4δ1, then these cells were separately stained with APC-conjugated anti-TCRγδ and PE-conjugated anti-TCRαβ mAbs and analyzed by flow cytometry. The percentage of positive cells for each quadrant is indicated. (TCR, T-cell receptor; CDR, complementary determining region; TIL, tumor-infiltrating lymphocyte.)