Figure 4
From: Activation of Proteinase 3 Contributes to Nonalcoholic Fatty Liver Disease and Insulin Resistance
NE−/−/CG−/− and NET−/−/PR3−/− mice have reduced inflammation after a high-fat diet. (A) Representative images of immunostaining of the macrophage marker F4/80 in white adipose tissue of wild-type, NE−/−/CG−/−and NE−/−/PR3−/− mice. (B) Average number of macrophages per field. (C) Average number of CLS per field. Secretion of (D) IL-6 and (E) KC by white adipose tissue explants. (F) Relative mRNA expression of IL-1β, Cd68, IL-6, Mcp-1 and IL-1Ra in liver. WT, wild-type; HFD, high-fat diet; CLS, crown-like structures; *significant difference p ≤ 0.05; **significant difference p ≤ 0.01 ***significant difference p ≤ 0.001; NS, nonsignificant.