Figure 1

Cerulein or L-arginine inhibiting autophagy flux correlates with trypsinogen activation in pancreatic acinar cells. (A, B). AR42J cells were treated with 200 nmol/L cerulein (A) or 10 mmol/L L-arginine (B) for 22 h followed by chloriquitone (CQ) or normal saline treatment (Control) for two hours. The levels of LC3II, p62 and ubiquitination (Ub) were next analyzed using the Western blotting technique. The graphs provided are quantified using ImageJ as means ± SD of three independent sets of experiments. Student t test was used. ***P < 0.001. (C-F) AR42J cells were treated with 200 nmol/L cerulein (C, E) or 10 mmol/L L-arginine (D, F) for indicated durations. The levels of LC3, p62, beclin1 and Ub were next analyzed using the Western blotting technique (C, D), and the corresponding graphs (right line graphs) were quantified using Image J from two independent sets of experiments (C, D). Error bars represent SD. The relative trypsin activity was evaluated by the fluorescence intensity. Student t test was used as statistical analysis from three independent sets of experiments (E, F).