Figure 2

Spautin-1 reduces impaired autophagy and inhibited trypsinogen activation in pancreatic acinar cells with cerulein or L-arginine treatment. (A, B) AR42J cells were treated with normal saline (NS), 200 nmol/L cerulein (A) or 10 mmol/L L-arginine (B) in the presence or absence of 10 µmol/L spautin-1 for 24 h. The trypsin activity was next analyzed by the fluorescence intensity. Data from three independent experiments were tested Student t test and shown as means ± SD. (C–F) AR42J cells with indicated treatment were lysed and were analyzed by Western blotting with anti-LC3, anti-p62, antibeclin1, Ub and antitubulin antibodies. The relative levels of LC3II, p62 and beclin1 expression were are quantified using Image J and exhibited as the bar graphs from three independent sets of experiments (C, E) and line graphs from two independent sets of experiments (D, F). Error bars represent SD. Student t test was used *P < 0.05, **P < 0.01, ***P < 0.001.