Figure 3

Spautin-1 ameliorates the pathogenesis of acute pancreatitis induced by cerulein or L-arginine in mice. (A–D) Cerulein-induced mice model with acute pancreatitis. Spautin-1 was injected half an hour before the first injection of cerulein. The mice were sacrificed nine hours after the last cerulein injection for analyzing serum amylase, lipase and TNFα levels (A), histopathological change (C) and the levels of p65 (B), LC3II, p62, beclin1 and Ub (D) in the pancreatic tissues. The data from serum analyses were tested using one-way ANOVA with LSD corrections and shown as means ± SD. (E-H) L-arginine-induced mice model with acute pancreatitis. Spautin-1 was injected 30 min before the first injection of L-arginine. The mice were sacrificed nine hours after the last L-arginine injection for analyzing serum amylase, lipase and TNFα levels (E), histopathological change (G) and the levels of p65 (F), LC3II, p62, beclin1 and Ub (H) in the pancreatic tissues. The data from serum analyses were tested using one-way ANOVA with LSD corrections and shown as means ± SD. The ratios between the level of relative protein and tubulin are listed below the band.