Figure 4

TGFβ-induced MRTF-A nuclear localization is ROCK-dependent. (A) The presence of Y-27632 (20 µM) prevented nuclear localization of MRTF-A in response to TGFβ (4 ng/mL) treatment for 48 h. Fixed explants were costained for MRTF-A and αSMA and mounted in medium with DAPI to colocalize nuclei. (B) Quantification of the ratio of nuclear-to-cytoplasmic MRTF-A signal by measuring intensity of fluorescence in images as described in Materials and Methods. Data expressed as means ± SEM from three separate experiments. Scale bar and insets = 100 µm (A).