Figure 6
From: Matrix Metalloproteinase-8 Augments Bacterial Clearance in a Juvenile sepsis Model
E. coli adherence to NETs of MMP8 null and wild-type animals. (A) Qualitatively, WT animals demonstrated frequent adherence of fluorescent-labeled bacteria to NETs. (B) By quantitative image analysis, the majority of bacteria (spheres) in WT nets were within 1µm of the DNA surface (green). Bacteria co-localized with DNA are labeled magenta and non-co-localized bacteria as aqua. (C) MMP8 null animals demonstrate less frequent localization of bacteria with NETS. (D) Non-co-localization of bacteria was more frequent in MMP8 null samples. (E) The bacteria in MMP8 null animals (n = 10) were about 50% less likely to be associated with DNA compared with the bacteria in WT animals (n = 10). *p < 0.05 by 2-tailed sum rank test. All data reported as mean ± SEM.