Figure 5

α7nAChR expression does not influence the severity of acute DSS colitis. α7nAChR^−/− and α7nAChR^+/+ mice were subjected to DSS-colitis. (A) Body weight and (B) stool consistency were scored daily. Statistical significance was determined using a multivariate linear model after a post-hoc Bonferroni-Holm correction for body weight changes and stool consistency score (ns, not significant). (C) Fourteen days after DSS treatment, mice were sacrificed to measure colon length and spleen weight. (D) Inflammatory gene expression was quantified via q-PCR in colonic tissue. Gene expression levels are shown relative to rpl32 mRNA expression. Data expressed as mean ± SEM, are representative of three independent experiments. Statistical significance was determined with the unpaired Student t-test for colon length, spleen weight, gene expression level and flow cytometry analysis (ns, not significant).