Figure 3

Hemopexin gene therapy increases hemopexin expression in livers and plasma of sickle mice. NY1DD (A, C and D) and Townes-SS (B, E and F) mice were hydrodynamically infused with lactated Ringer’s solution (LRS), Sleeping Beauty-100X transposase (SB100X) + luciferase (Luc), or SB100X + wt-Hpx (n = 4). Liver and plasma samples were collected 4 wks later after measurement of heme-induced stasis and run on western blots. Liver microsomes from NY1DD (A) and Townes-SS (B) mice were immunostained for hemopexin (Hpx) (68 kDa) and GAPDH (32 kDa). Plasma samples from treated NY1DD mice and untreated C57BL/6 control mice (C) and treated Townes-SS mice and untreated Townes-AA control mice (E) were immunostained for Hpx and IgG (155 kDa). Relative Hpx band intensities are presented in (D) and (F). In (D), #p < 0.05 versus C57BL/6 and *p < 0.01 versus C57BL/6. In (F), ^#p < 0.05 Townes-SS-wt-Hpx versus Townes-SS-LRS or Townes-SS-Luc and *p < 0.01 Townes-SS-LRS or Townes-SS-Luc versus Townes-AA.