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Figure 3 | Molecular Medicine

Figure 3

From: Mutations in the Na+/Citrate Cotransporter NaCT (SLC13A5) in Pediatric Patients with Epilepsy and Developmental Delay

Figure 3

Western blots. (A) Total cell protein. Protein lysates from COS-7 cells transfected with empty vector, pcDNA, wild-type NaCT (WT) and mutants. Duplicate blots were probed with anti-NaCT (top) and GAPDH antibodies (bottom). Size standards (kDa) are shown at left. (B) Cell surface biotinylated proteins from HEK-293 cells treated with membrane-impermeant sulfo-NHS-LC-biotin followed by streptavidin beads. The HEK-293 cells were used for biotinylations because the signal was stronger than with COS-7 cells.

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Molecular Medicine

ISSN: 1528-3658