Figure 5

Effects of cotransfection of wild-type NaCT with vector plasmid (pcDNA) or mutant transporters. (A) Citrate transport activity. All groups were transfected with the same amount of wild-type NaCT DNA and a second plasmid. Activity is expressed as a percentage of the NaCT + pcDNA control group. All counts were corrected for background counts determined from cells transfected only with pcDNA plasmid. Bars are means ± SEM, n = 3−5, or range, n = 2 (DelG). * denotes p < 0.05 relative to the NaCT + pcDNA group. (B) Western blots. Duplicate blots were probed with anti-NaCT (top) and anti-GAPDH (bottom) antibodies. Groups were the same as for panel (A). The samples in the lane next to the size standards (labeled none) were transfected only with pcDNA, the other samples had two plasmid DNAs (NaCT and a second plasmid). All cells were transfected with the same total amount of DNA.