Figure 11

Effect of YD0171 on cell viability and cell death in HCT116 cells. Cells were treated with YD0171 (100),300 or 1 mmol/L) for 48 h, followed by flow cytometry analysis of phospholipid redistribution by the annexin V/propidium iodide assay. Samples were analyzed for green fluorescence (FITC) and for red fluorescence (PI) by flow cytometry. Typical profiles are represented in the left panels. The bar graph on the right side shows the relative proportions of the live, necrotic, early apoptotic and late apoptotic cell populations; data are shown as mean ± SEM; n = 6.