Figure 13

Inhibition of cell proliferation and H₂S production by YD0171 in HCT116 cells. (A) Cells were treated with various concentrations of AOAA or YD0171 in 96-well xCELLigence plates, and proliferation was monitored for 48 h. Cell proliferation is shown over time for AOAA (top panel) and YD0171 (bottom panel) treated cells from one representative experimental run. Note that AOAA only inhibited cell proliferation at the highest concentration tested (1 mmol/L), while the inhibitory effect of YD0171 on cell proliferation was already apparent at 100–300 µmol/L. (B) Summary data (mean ± SEM) of n = 6 independent experiments testing the effect of AOAA and YD0171 in cultured HCT116 cells. Cell proliferation at 48 h is expressed as 100%. YD0171 inhibited the proliferation with an IC₅₀ of 200 µmol/L, while the IC50 of AOAA was 500 µmol/L.