Table 2 Metabolomic effects: modulation of selected cellular energetics-related metabolites in HCT116 cells by YD0171 and AOAA treatment (1 mmol/L, 24 h). Numbers represent fold changes in metabolite concentrations; red background indicates an increase, green background indicates a decrease in the concentration of the metabolite over vehicle-treated control.

TCA cycle, oxidative phosphorylation

Citrate

1.52

1.95

Isocitrate

1.66

3.02

α-Ketoglutarate

2.04

3.13

Succinylcarnitine

0.38

0.38

Succinate

0.42

0.56

Fumarate

0.12

Malate

0.12

2-Methylcitrate/homocitrate

1.39

1.19

Acetylphosphate

1.49

1.53

Phosphate

1.13

1.07

Glycolysis, gluconeogenesis, pyruvate metabolism

Glucose

1.82

1.47

Glucose 6-phosphate

1.53

1.55

Fructose-6-phosphate

0.64

0.69

Isobar: fructose 1,6-diphosphate, glucose 1,6-diphosphate, myo-inositol 1,4 or 1,3-diphosphate

2.56

2.06

2,3-Diphosphoglycerate

0.84

0.62

Dihydroxyacetone phosphate

1.87

0.86

3-Phosphoglycerate

1.89

2.36

Phosphoenolpyruvate

3.07

3.31

Pyruvate

4.91

3.83

Lactate

0.53

0.62

Glycerate

1.07

0.85

Pentose phosphate pathway

6-Phosphogluconate

1.55

1.66

Ribose 1-phosphate

0.38

0.31

5-Phosphoribosyl diphosphate

3.42

1.22

Sedoheptulose-7-phosphate

1.77

2.08

Creatine metabolism

Creatine

1.13

1.19

Creatinine

0.81

0.88

Creatine phosphate

1.59

1.86