Figure 2
From: Shock Wave Therapy Enhances Angiogenesis through VEGFR2 Activation and Recycling
Shock wave (SW)-induced angiogenesis in vitro. (A) Representative images of tube formation assay with basal medium (BM, defined as serum- and growth factors-free) and complete medium (CM). (B) Parameters of tube formation assay, including tube length, branch point and the number of loops (n = 5 in each group). (C) Representative images of tube formation assay in control (CON) group and a dose-dependent increase of SW treatment in HUVECs (scale bar, 200 µm). (D) Parameters of tube formation assay, including tube length, branch point and the number of loop (n = 5 in each group). P for trend in those parameters showing a dose-dependent increased order. (E) Control (CON) and SW-treated HUVECs labeled with PKH67 (green) and PKH26 (red), respectively. Representative images of fluorescence signals (scale bar, 100 µm). Merged image with magnification indicated by yellow line showing amalgamation in tube formation assay. Data shown as mean ± S.D. *P < 0.05 determined by Student t test.