Figure 6
From: Shock Wave Therapy Enhances Angiogenesis through VEGFR2 Activation and Recycling
Importance of vascular endothelial growth factor receptor 2 (VEGFR2) in shock wave (SW)-induced angiogenesis. (A) Treatment with low-dose (5 µmol/L) (SW + Low SU5416) and high-dose (15 µmol/L) (SW + High SU5416) of SU5416 (VEGFR2 kinase inhibitor) in SW-treated HUVECs during tube formation assay. Morphology of tube-like structure at 6 h post seeding. (B) Parameters of tube formation assay, including tube length, branch point and the number of loops (n = 7 in each group). (C) Three different small interfering RNAs (siRNAs) for VEGFR2 silencing in HUVECs, evaluated by Western blot. (D) No.1 and No. 2 siRNAs-transfected HUVECs applied in tube formation assay, and (E) Quantification (n = 6 in each group). Data shown as mean ± S.D. ***P < 0.0005, *P < 0.05 determined by Student t test.