Figure 7
From: Shock Wave Therapy Enhances Angiogenesis through VEGFR2 Activation and Recycling
Expression of vascular endothelial growth factor receptor 2 (VEGFR2) in shock wave (SW)-induced angiogenesis. (A) Protein expression of VEGFR2 in HUVECs with (SW) and without (CON) SW treatment determined by Western blot (n = 4 in each group). (B) Expression of VEGFR2 mRNA in HUVECs at the end of 28 h without (CON) and with SW treatment determined by RT-qPCR (n = 3 in each group). (C) Illustration showed the endocytic route of endothelial VEGFR2 from early endosome to recycling endosome and ship back to the plasma membrane through Rab11a. Rab11a silencing in HUVECs by three different small interfering RNAs (siRNAs) evaluated by Western blot. (D) Tube formation assay posttransfection of scramble (Scr), No.2, and No.3 siRNAs with SW compared with those in the control (CON) without SW treatment. (E) Changes in parameters of tube formation assay, including tube length, branch point and the number of loops (n = 5 in each group) post Rab11a silencing with No.2 and No.3 siRNAs. Data shown as mean ± S.D. *P < 0.05 determined by Student t test.