Three Interacting Genomic Loci Incorporating Two Novel Mutations Underlie the Evolution of Diet-induced Diabetes

Table 1 Cosegregation analysis for representative microsatellite markers within the genomic loci on RNO4, −11 and −13.

			Genotype	AUC			ANOVA		LSD test
			Genotype	AVG	SEM	N	F	P	SS vs RR/SR
Single locus effect		RNO4	SS	40366	1969	44	46.02	<0.0001	P < 0.0001
		locus	SR/RR	30511	533	188
		RNO11	SS	35896	1455	60	11.64	0.0006	P = 0.0008
		locus	SR/RR	31154	646	172
		RNO13	SS	35651	1506	52	9.38	0.0025	P = 0.0025
		locus	SR/RR	31256	639	171
Two loci effect		RNO4	RNO11	AVG	SEM	N	F	P	P value
	1	SS	SS	47996	2962	12			1 vs 2 0.0054
	2	SS	SR/RR	37505	2293	32	9.52	0.0002	1 vs 3 <0.0001
	3	SR/RR	SS	32871	1355	32			2 vs 3 0.6510
		RNO4	RNO13	AVG	SEM	N	F	P	P value
	1	SS	SS	48558	8151	6			1 vs 2 0.0278
	2	SS	SR/RR	38408	1787	37	6.09	0.0034	1 vs 3 0.0016
	3	SR/RR	SS	33967	1181	46			2 vs 3 0.0543
		RNO11	RNO13	AVG	SEM	N	F	P	P value
	1	SS	SS	39222	3451	13
	2	SS	SR/RR	34976	1658	47	0.95	0.3902	—
	3	SR/RR	SS	34461	1726	39
Three loci effect	RNO4	RNO11	RNO13	AVG	SEM	N	F	P	P value
Three loci effect	SR/RR	SR/RR	SR/RR	28592	531	105
1	SS	SR/RR	SR/RR	35135	1757	27			1 vs 2 0.0072
2	SS	SS	SR/RR	47244	3359	10	5.03	0.0115	1 vs 3 0.0628
3	SS	SR/RR	SS	46959	12431	4			2 vs 3 0.9669
1	SR/RR	SS	SR/RR	31661	15044	37			1 vs 2 0.1097
2	SR/RR	SS	SS	36944	2850	11	10.87	0.0001	1 vs 3 <0.0001
3	SS	SS	SR/RR	47244	3359	10			2 vs 3 0.0158
1	SR/RR	SR/RR	SS	33032	1252	35			1 vs 2 0.2592
2	SR/RR	SS	SS	36944	2850	11	3.82	0.0291	1 vs 3 0.0106
3	SS	SR/RR	SS	46959	1243	4			2 vs 3 0.0900
	SS	SS	SS	51754	9959	2

Data are presented for single locus, two loci and three loci effects, as the mean (AVG) of the area under the curve (AUC) after 1 month of DD, grouped by genotypes RR, SS and SR. Group averages were compared by one-way ANOVA, providing F and p values; post hoc analysis was performed using the least significant difference (LSD) test. SS, homozygous for CDs/y; SR, heterozygous homozygous for CDr/y.

ISSN: 1528-3658