Figure 1

Effects of A1AT-0 and A1AT-LA on LPS-induced IL-1β precursor and IL-1β released. Human neutrophils (5 × 10⁶) were incubated for 5 h in either medium alone or medium containing LPS (20 ng/mL) in the absence or presence of A1AT-0 (1 mg/mL) or A1AT-LA (1 mg/mL). Released IL-1β levels (A), IL-1β gene expression (B), cell-associated profile, and levels of IL-1β protein were determined by western blotting (C) and ELISA (D). GUSB and GAPDH were used as housekeeping genes in qPCR experiments. Box plots represent data from 5 individual blood donors; n = number of replicates for each experiment. Blot is representative of 3 independent donors. P value indicates significant differences compared with the values seen in LPS-activated cells.