Figure 7

Effects of A1AT-0 and A1AT-LA on LPS-induced NE and PR3 expression and protein profile. Human neutrophils (5 × 106) were incubated for 5 h in either medium alone or medium containing LPS (20 ng/mL) in the absence or presence of A1AT-0 (1 mg/mL) or A1AT-LA (1 mg/mL). mRNA expression of PRTN3 (A) and ELANE (B) were analyzed by real-time qPCR as described in Materials and Methods. GAPDH and HPRT were used as housekeeping genes. Box plots represent data from 5 individual donors; n = number of replicates for each experiment. P value indicates significant differences compared with the values seen in control cells. Cell lysates were analyzed for A1AT (C), PR3 (D) and NE (E) protein profile characterization by 12.5% SDS-PAGE following western blotting. M-molecular size marker (kDa). Each blot is representative of 3 independent donors.