Figure 4

TO reverses and overcompensates for many of the gene expression changes induced by surgical DH. (A) After false discovery correction and using a p < 0.1 level of significance and comparing each group to the others, there were very few genes with significantly different levels of expression among the 13,687 genes analyzed. The exception to this was an increased number of differentially expressed genes between DH and DHTO (687 genes). As expected, PND1 rabbit lung had more differentially expressed genes than fetal lung time points. Note the log10 scale. (B) Hieratical clustering of gene expression profiles demonstrated that TO was more similar to control than DHTO and that DHTO was more like control (Ctrl) than DH. (C) Gene Expression Dynamics Inspector (GEDI) was used to generate average gene expression heat maps for each fetal lung group. DHTO had higher expression of the genes grouped in region 1 and lower levels of gene expression in region 2 compared with control, DH and TO. In region 3, DH had reduce levels of gene expression compared with control and DHTO had higher expression levels of these genes.