Figure 3
Effect of antioxidant treatment on the recovery of mitochondrial biomarkers. (A) Citrate synthase activity (expressed as nmol × (min × mg protein)−1) was evaluated in untreated (media only), vehicle-treated (DMSO), and antioxidant-treated cells (n = 5 and n = 3, for controls and premutation, respectively; see Table 1) after 48 h. The 95% CIs for each treatment were performed with control values (C2–C3, C16, C18–C19). Values in red indicate below the 95% CI; in green above, and not colored within the limits of the 95% CI. (B) Representative image of a Western blot run with cells (two controls and three premutation) treated with media (none or N), DMSO (D), Ebselen (E, in DMSO) and Trolox (T, in DMSO) for 48 h and evaluated for VDAC, COXIV and β-actin (loading control) protein expression. (C) Cells from premutation donors were treated for 48 h with quercetin, EGCG or vehicle DMSO and Western blots evaluated VDAC protein expression (the doublet could be attributed to alternative spliced products).