Figure 4
From: Interrelationship between Changes in the Amyloid β 42/40 Ratio and Presenilin 1 Conformation
Intracellular calcium level upon Aβ treatment. (A) The relative levels of intracellular calcium in primary neurons were determined using ratiometric Indo-1 dye. The graph presents the increase in the calcium level upon treatment with Aβ peptides at increasing Aβ42/40 ratio. n = 234 (vehicle), n = 243 (1:9), n = 271 (3:7), n = 263 (4:6), n = 219 (10:0), n = total number of neurons analyzed from three independent experiments, mean ± SEM, two-tailed unpaired Student t test. (B) Correlation between PS1 conformational changes (FRET efficiency) and intracellular calcium levels in primary neurons treated with vehicle or Aβ42 to Aβ40 at 1:9; 3:7; 4:6 or 10:0 ratios. Pearson’s X-square test. Aβ — amyloid β; PS1 — presenilin 1; FRET — Förster resonance energy transfer.