Figure 4

Effect of PfHz and heat shock treatment on NF-κB translocation into the nucleus. Peripheral blood mononuclear cells (2.5 × 10⁶) were incubated for 2 h at either 37°C (physiological) or 42°C (heat shock treatment) and stimulated with LPS for 1 h. Cells were treated with PfHz (10 µg/mL) and incubated for different time points (2 to 48 h). (A) Nuclear extracts were prepared after harvesting cells and equal amounts of protein were loaded on 4–20% SDS-PAGE to determine the amount of nuclear translocated NF-κB by immunoblotting. In addition, a nuclear marker (histone protein) was quantified to account for protein loading (control). (B) Densitometry analysis after normalizing the respective bands against histone (n = 3); error bars represent SEM.