Figure 7
Effect of PfHz and glutamine on NF-κB translocation into the nucleus. (A) Peripheral blood mononuclear cells (2.5 × 106) were incubated with LPS for 1 h prior to the addition of Gln (0.5, 2.0 and 4.0 mM, pretreated 30 min prior to adding PfHz) for different time points. Nuclear extracts were prepared after harvesting cells at stipulated time points and equal amount of protein was loaded on 4–20% SDS-PAGE to determine the amount of nuclear translocated NF-κB protein by immunoblotting. In addition, a nuclear marker (histone protein) was quantified to account for protein loading (control). (B) Densitometry analysis after normalizing the respective bands against histone (n = 3); error bars represent SEM.