Figure 2
From: Development of a Zebrafish Sepsis Model for High-Throughput Drug Discovery
Use of transgenic zebrafish lines and microangiography contrast agents help visualize vascular leakage. The use of transgenic zebrafish lines (Flk1: GFP and Kdrl: mcherry) together with injection of microangiography contrast agents like QD605 and FITC-dextran, respectively, help visualize LPS-induced vascular leakage in 3DPF zebrafish larvae. (A, B) While QD605 are useful in detecting major leaks at the region below the PCV (an average of 3 trials of 9–14 larvae per treatment), (C, D) FITC-dextran is better suited for visualizing minor leaks through the ISVs and the subsequent accumulation of the leaked out dextran at the tail fin (an average of 3 trials of 10–15 larvae per treatment). DLAV: dorsal longitudinal anastomotic vessel; ISA: intersegmental artery; ISV: intersegmental vein; PCV: posterior cardinal vein; DA: dorsal aorta