Figure 2

Dietary soybean oil diet increased hepatic lipid peroxidation, but only soybean oil diet + cholesterol provoked oxidative stress and apoptosis in mouse livers. Mice received diets as described in Figure 1. (A) Lipid peroxidation: malondialdehyde relative to protein content in liver homogenates. (B,C) Oxidative stress: hyperoxidized peroxiredoxins (Prx-SO₃, B) were determined in liver lysates by Western blot analysis with a specific antibody and normalized to Fast Green staining, which was verified on the same Western blot membrane as a loading control. Carbonylated proteins were verified by oxyblot with (C) ponceau S staining as a loading control. (D) Apoptosis: DNA damage in apoptotic cells was visualized by TUNEL staining. Values represent (A,B) means ± standard error of the mean of 5 mice per group or (C,D) representative photographs. Statistics: (A,B) One-way analysis of variance with Dunnett’s post hoc test or, where appropriate, Tukey’s post hoc test for multiple comparisons, *p <0.05,