Figure 2

Extracellular HSP27 mediates postischemic inflammatory response and functional injury in aging hearts. Hearts from adult and aging mice were subjected to global ischemia/reperfusion (I/R: I 20 min/R 60 min). Hearts were treated with a polyclonal antibody against heat shock protein 27 (HSP27 Ab, 1.0 µg/mL) or control IgG (IgG, 1.0 µg/mL) for 10 min before ischemia and for 20 min after initiation of reperfusion. A) Anti-HSP27 improved cardiac functional recovery in both adult and aging hearts, but had a greater effect in aging hearts. Data are expressed as mean ± SE. n = 7; ^aP < 0.05 versus adult IgG + I/R; ^bP < 0.05 versus aging IgG + I/R. B) Anti-HSP27 reduced myocardial cytokine levels in adult and aging, and the effect was greater in aging hearts. Data are expressed as mean ± SE. n = 7; ^aP < 0.05 versus age-matched control; ^bP < 0.05 VS. adult I/R or IgG + I/R; ^cP < 0.05 versus age-matched IgG + I/R.