Figure 4
From: IL-37 Suppresses MyD88-mediated Inflammatory Responses in Human Aortic Valve Interstitial Cells
IL-37 attenuates the inflammatory responses to TLR2 stimulation, but has no effect on the responses to TLR3 stimulation. AVICs were treated with poly(I:C) (2.5 µg/mL) or Pam3CSK4 (0.1 µg/mL) for 24 h in the presence and absence of IL-37 (0.1 and 1.0 ng/mL). (A) Representative immunoblots and densitometric data show that poly(I:C) and Pam3CSK4 upregulated ICAM-1 production. Whereas IL-37 reduced ICAM-1 production induced by Pam3CSK4, it failed to inhibit the effect of poly(I:C) on ICAM-1 production. (B) Cytokine data show that cells produced higher levels of cytokines following stimulation with either poly(I:C) or Pam3CSK4. IL-37 only reduced cytokine production in cells exposed to Pam3CSK4. Data are mean ± SE. n = 6 experiments using cell isolates from distinct donor valves; *P <0.05 versus control; #P <0.05 versus Pam3CSK4 alone.