Figure 1

Analysis of CCDC80 and CD14 protein content in SAT and VAT depots from lean and obese patients and CCDC80 protein in human plasma. (A) Western blot analysis of SAT and VAT extracts (20 µg protein). Membranes were hybridized with antibodies against CCDC80, CD14 and FAA. A representative blot is shown. Bands were quantified and the ratio of intensities between CCDC80 or CD14 and control FAA was calculated. (B) Relative CCDC80 protein content. Data are expressed in arbitrary units and are means ± SEM from 5 samples. #P < 0.05 between different depots in lean patients; *P < 0.05 between VAT depots in obese versus lean patients. (C) Western blot analysis of 1 µl of human plasma and 0.8 ng of purified CCDC80 protein (50 kDa fragment). The membrane was hybridized with an antibody against CCDC80. (D) Relative CD14 protein content. Data are expressed in arbitrary units and are means ± SEM from 5 samples. #P < 0.05 between different depots in obese patients; *P < 0.05 between VAT depots in obese versus lean patients.