Figure 4
Intracellular signaling pathway activation in isolated perfused rat hearts exposed to I/R. (A) Representative Western blots (WB) showing α7nAChR protein content in rat LV of in vivo and ex vivo models of I/R injury treated with GTS21 or vehicle; PC, perfusion control; no statistical differences among groups (n = 4–5/group). (B) Quantitation of immunoblots in (A) showing α7nAChR normalized to GAPDH. (C) Representative immunoblots for JNK, p38MAPK, AKT and their phosphorylated isoforms in I/R perfused LVs treated with vehicle or GTS21. (D) Quantitation of the WB shown as bar graphs for phospho-JNK to total JNK and p-p38MAPK to total p38MAPK ratios normalized to PC. Values are mean ± SE; *p < 0.01 versus all groups. Statistical significance determined by one-way ANOVA.