Figure 6

Effects of RAGE-aptamer or NAC on (A) ROS generation, (B, C) proliferation, (D, E) cyclin D1 and p27 protein levels, (F, G) VEGF and (H, I) MCP-1 mRNA levels in AGE-exposed G361 melanoma cells. (D, E) Each upper panel shows the bands of Western blot analysis for cyclin D1, p27 and β-actin, respectively. Quantitative data are shown in each lower panel. Data were normalized by the intensity of β-actin-derived signals and related to the value obtained with nonglycated BSA alone. (A) N = 6 for each group. (B, C, E) N = 3 for each group. (D) N = 5 for each group. (F–I) N = 9 for each group. **, p < 0.01 compared with the control value with nonglycated BSA alone. # and ##, p < 0.05 and p < 0.01 compared with the value with AGEs alone, respectively.