Figure 7
From: RAGE-aptamer Attenuates the Growth and Liver Metastasis of Malignant Melanoma in Nude Mice
Effects of RAGE-aptamer on (A) ROS generation, (B) proliferation, (C) VEGF, (D) VCAM-1 and (E) MCP-1 mRNA levels in, (F) THP-1 cell adhesion to, and (G) tube formation of, AGE-exposed HUVECs. (A) Superoxide generation was evaluated by the fluorescence intensity of DHE staining. (B) Proliferation was determined by (3H)thymidine incorporation into HUVECs. (C–E) Total RNA was transcribed and amplified by real-time PCR. Data were normalized by the intensity of β-actin-derived signals and related to the value obtained with nonglycated BSA alone. (G) Length of tube-like structures was measured. Upper panels show the representative micro-photographs. (A) N = 3 for each group. (B) N = 4 for each group. (C) N = 6 for each group. (D, E) N = 3 for each group. (F) N = 6 for each group. (G) N = 3 for each group. * and **, p < 0.05 and p < 0.01 compared with the value with AGEs alone, respectively.