Oligoclonality of CD8+ T Cells in Breast Cancer Patients

Table 3 TCR Vβ18 sequences of CD8⁺ T cells of breast cancer patients

Patient (PBMC)	V β18	NDN	J	C	CDR3 length
H.H.	CASS	SRTSGGQG	DTQY FGPGTRLTVL (2.3)	ED	13 aa^a
V.V.	CASS	PPGLSD	TEAF FGQGTRLTVV (1.1)	ED	11 aa^a
M.T.	CASS	PSLGRD	EQY FGPGTRLTVT (2.7)	ED	10 aa^a
P.V.	CASSP	PLGF	TEAF FGQGTRLTVV (1.1)	ED	10 aa^b
H.E.	CASSP	RGYYD	IQY FGAGTRLSVL (2.4)	ED	10 aa^a
J.W.	CASS	VGEG	TEAF FGQGTRLTVT (1.1)	ED	9 aa^a
I.D.	CASSP	WPSG	TQY FGPGTRLTVL (2.3)	ED	11 aa^a
M.T.	CASS	PSLGRD	EQY FGPGTRLTVT (2.7)	ED	10 aa^a
P.V.	CASSP	PAGT	EQF FGPGTRLTVL (2.1)	ED	9 aa^b

^aSequence obtained by directly sequencing DNA fragments eluted from those domiant CDR3 bands on acrylamide gel.
^bSequence obtained by bacterial cloning method. cDNA fragment for each TCR Vβ gene family was synthesized and cloned according to the procedures described in Materials and Methods. More than 20 clones were picked and sequenced. The sequence obtained from more than 50% of all clones was designated the dominant sequence.

ISSN: 1528-3658