Tissue distribution of the ET-1/AngII receptor mRNA. RNA blot analysis of different adult rat tissue total cellular RNAs (5 µg) is presented (A). Lane 1: kidney, lane 2: brain, lane 3: cardiac left ventricle, lane 4: cardiac right ventricle, lane 5: atria, lane 6: aorta, lane 7: adrenal gland, lane 8: lung, lane 9: liver. The RNA blot was hybridized to an ET-1/AngII receptor cDNA probe encompassing nucleotides 2520–3274 of the full-length cDNA (Fig. 1) as previously described (41). Nondegradation of RNA samples and relative amounts are directly documented in the ethidium-bromide picture presented in parallel (B). 28S and 18S ribosomal RNAs are marked by arrowheads.