Fig. 2

Effect of Bcr-Abl expression on the maturational profile of the stem cell compartment. The compartments within the CD34+Lin− stem cell population are shown in order of increasing maturity. (A) The proportion of cells within each compartment for normal bone marrow (NBM; n = 3) and chronic myeloid leukaemia (CML; n = 5), including one bone marrow sample. Results are shown as the mean ± standard error of the mean (SEM) of the data depicted in Table 1. A Students t-test was applied to all the data and p-values are shown. Significant differences are marked (*). (B) The fold expansion during maturation was calculated by the ratio of cells in the more mature compartments (CD34+Lin-Thy+Rh-123hi, CD34 + Lin-Thy-Rh-123lo and CD34+Lin-Thy-Rh-123hi) to those in the initial compartment (CD34+Lin-Thy+Rh-123lo). (C) The proportion of cells in cycle for each population, as determined by Hoechst 33342 staining. Results are shown as the mean ± standard error of the mean (SEM) for the data depicted in Table 1. Rh-123lo and Rh lo, low levels of rhodamine-123; Rh-123hi and Rh hi, high levels of rhodamine-123.