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Table 2 Transient insertion of infectious wires into brains of indicator mice

From: Transmission of Scrapie by Steel-surface-bound Prions

Inoculation Sick/Total Incubation Time ± s.d. (days)
Wires infected by exposure to scrapie brain   
(a) Transient insertion into indicator mice   
30 min 4/4$ 94 ± 10
120 min 2/2# 100 ± 18&
(b) Permanent insertion into indicator mice   
Wires not previously inserted 3/3 71 ± 2
Wires after transient insertion for: 30 min 4/4 71 ± 3
120 min 5/5 68 ± 1
(c) Controls   
Wires exposed to brain homogenate 6/6 76 ± 3
Brain homogenate (1%, 0.03 ml) 3/3 69 ± 3
  1. Infectious wires were prepared by insertion for 5 min into the brain of C57Bl6 × 129Sv mice culled 121 days after i.c. inoculation with RML and washed with 50 ml PBS 5 times for 10 min. Infectious wires were inserted into brains of 6 deeply anaesthetised Tga20 indicator mice for the times indicated. The recovered wires were washed with 1 ml PBS and implanted into Tga20 indicator mice. As controls, wires incubated with centrifuged 10% homogenate (6.8 log LD50 units/ml) of the same brain and the homogenate itself were introduced into indicator mice.
  2. $Two of 6 mice died on the day of the intervention.
  3. #Four of 6 mice died within a day of the intervention.
  4. &Incubation times were 87 and 113 days.