- The mRNA of the depicted genes was at least twofold differentially regulated in Rab31 overexpressing compared to vector control cells in two independent biological replicates applying the Human TGF-β/BMP RT2 Profiler PCR Array (Qiagen). The genes with grey background were further analyzed by using Roche Universal Probe Library-based qPCR assays, whereby the genes in bold were validated as differentially expressed genes